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You can get them all in a single playthrough, and if you miss some along the way, you can always go back through Chapter Select and get them later. Keep in mind that there have been some minor tweaks made to the original placements of the collectibles. Most of them will fall into your lap as you progress through the story, but a few others may require some exploring.
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You can play this mode once the main story is completed, and it features its own set of collectibles and optional conversations. The Last of Us: Left Behind Remake allows players to explore Ellie’s life before she met Joel, which also serves to bridge a plot hole created when Ellie leaves the main story to get medical supplies.
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